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HelpTest Code ENAE Antibody to Extractable Nuclear Antigen Evaluation, Serum
Reporting Name
Ab to Extractable Nuclear Ag Eval,SUseful For
Evaluating patients with clinical features suggestive of antinuclear antibody (ANA) associated connective tissue disease. May also be indicated in patients who test negative for ANA and have features of Sjogren syndrome and idiopathic inflammatory myopathies
Profile Information
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| SSA | SS-A/Ro Ab, IgG, S | Yes | Yes |
| SSB | SS-B/La Ab, IgG, S | Yes | Yes |
| SM | Sm Ab, IgG, S | Yes | Yes |
| RNP | RNP Ab, IgG, S | Yes | Yes |
| SCL70 | Scl 70 Ab, IgG, S | Yes | Yes |
| JO1 | Jo 1 Ab, IgG, S | Yes | Yes |
Testing Algorithm
For more information see Connective Tissue Disease Cascade.
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
SerumSpecimen Required
Collection Container/Tube:
Preferred: Red top
Acceptable: Serum gel
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Specimen Minimum Volume
0.35 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 21 days | |
| Frozen | 21 days |
Special Instructions
Reference Values
SS-A/Ro ANTIBODIES, IgG
<1.0 U (negative)
≥1.0 U (positive)
Reference values apply to all ages.
SS-B/La ANTIBODIES, IgG
<1.0 U (negative)
≥1.0 U (positive)
Reference values apply to all ages.
Sm ANTIBODIES, IgG
<1.0 U (negative)
≥1.0 U (positive)
Reference values apply to all ages.
RNP ANTIBODIES, IgG
<1.0 U (negative)
≥1.0 U (positive)
Reference values apply to all ages.
Scl 70 ANTIBODIES, IgG
<1.0 U (negative)
≥1.0 U (positive)
Reference values apply to all ages.
Jo 1 ANTIBODIES, IgG
<1.0 U (negative)
≥1.0 U (positive)
Reference values apply to all ages.
Day(s) Performed
Monday through Saturday
Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
86235 x 6
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| ENAE | Ab to Extractable Nuclear Ag Eval,S | 90228-8 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| JO1 | Jo 1 Ab, IgG, S | 33571-1 |
| RNP | RNP Ab, IgG, S | 29958-6 |
| SCL70 | Scl 70 Ab, IgG, S | 47322-3 |
| SM | Sm Ab, IgG, S | 18323-6 |
| SSA | SS-A/Ro Ab, IgG, S | 33610-7 |
| SSB | SS-B/La Ab, IgG, S | 33613-1 |
Report Available
Same day/1 to 2 daysReject Due To
| Gross hemolysis | Reject |
| Gross lipemia | Reject |
| Gross icterus | OK |
| Heat-Treated | Reject |
Method Name
Multiplex Flow Immunoassay